Effects of the herbicide metolachlor and pond drying on growth and development of wood frog tadpoles (Lithobates sylvaticus)
Submitted to Environmental Toxicology and Chemistry

Simon Laporte, Département de biologie, Université de Sherbrooke, Sherbrooke, QC, Canada 
Dany Garant, Département de biologie, Université de Sherbrooke, Sherbrooke, QC, Canada 
Patrick Bergeron, Department of Biology and Biochemistry, Bishop’s University, Sherbrooke, QC, Canada 
*Corresponding author: patrick.bergeron@ubishops.ca

Goal: We used a mesocosm design to test the effects of metolachlor, drying and their combined effects on survival, on growth and on development of wood frog tadpoles.

List of file names:

Growth.csv

File containing body mass of tadpoles through out the growing season 

Date: Date at which a specific data was collected
Bin_id: Identity of each mesocosm 
Cattle_tank_id: Identity of each cattle tank holding specific mesocosms
Individu: Identity of a specific individual 
JJ: Day at which data was collected
Photo_id: Name of each picture
Mass: Body mass of an individual (g)
Developmental_stage: Developmental stage of tadpoles (1: no bud limbs, 2: Small bud limbs, 3: Visible legs, 4: Toe seperation, 5: Front bud limbs, 6: Metamorph)
Meto_level: Initial concentration of metolachlor (High = 80 µg/L, medium = 8 µg/L, low = 0.8 µg/L, nul = 0 µg/L) 
Drying_level: Drying rate (Rapid, medium, no-drying)
Mass_JJ16_17: Mean body mass of tadpoles in the mesocosm at the beginning of drying and metolachlor exposure (day 16)

Bodymass.csv

File containing body mass of metamorphs and the bodymass of tadpoles when we drained the mesocosms

Date: Date at which a specific data was collected
Bin_id: Identity of each mesocosm 
Cattle_tank_id: Identity of each cattle tank holding specific mesocosms
Individu: Identity of a specific individual 
JJ: Day at which data was collected
Photo_id: Name of each picture
Mass: Body mass of an individual (g)
Developmental_stage: Developmental stage of tadpoles (1: no bud limbs, 2: Small bud limbs, 3: Visible legs, 4: Toe seperation, 5: Front bud limbs, 6: Metamorph)
Meto_level: Initial concentration of metolachlor (High = 80 µg/L, medium = 8 µg/L, low = 0.8 µg/L, nul = 0 µg/L) 
Drying_level: Drying rate (Rapid, medium, no-drying)
Mass_JJ16_17: Mean body mass of tadpoles in the mesocosm at the beginning of drying and metolachlor exposure (day 16)

Larvalperiodduration.csv

File containing the capture date of each metamorphs

Date: Date at which a specific data was collected
Bin_id: Identity of each mesocosm 
Cattle_tank_id: Identity of each cattle tank holding specific mesocosms
Individu: Identity of a specific individual 
JJ: Day at which data was collected
Photo_id: Name of each picture
Developmental_stage: Developmental stage of tadpoles (1: no bud limbs, 2: Small bud limbs, 3: Visible legs, 4: Toe seperation, 5: Front bud limbs, 6: Metamorph)
Meto_level: Initial concentration of metolachlor (High = 80 µg/L, medium = 8 µg/L, low = 0.8 µg/L, nul = 0 µg/L) 
Drying_level: Drying rate (Rapid, medium, no-drying)
Mass_JJ16_17: Mean body mass of tadpoles in the mesocosm at the beginning of drying and metolachlor exposure (day 16)

Survival.csv

File containing the proportion of tadpoles and metamorphs that survived for each mesocosm from JJ0 till the day of drainage.

Bin_id: Identity of each mesocosm 
JJ: Date at which we drained the mesocosm
Survival_prop: Proportion of tadpoles and captured metamorphs when we drained the mesocosms. ((Number tadpoles + metamorphs)/50)
Meto_level: Initial concentration of metolachlor (High = 80 µg/L, medium = 8 µg/L, low = 0.8 µg/L, nul = 0 µg/L) 
Drying_level: Drying rate (Rapid, medium, no-drying)
Mass_JJ_16_17: Mean body mass of tadpoles in the mesocosm at the beginning of drying and metolachlor exposure (day 16)

Waterparameters.csv

File containing the water parameters measured in the mesocosms through out the experiment.

JJ: Day at which data was collected
Heure_debut: Time (hour) at which we started taking water temperature and concentrations of disolved oxygen in the mesocosms
Heure_fin: Time (hour) at which we ended measurements of water temperature and concentrations of disolved oxygen in the mesocosms
Bind_id: Identity of each mesocosm 
Cattle_tank_id: Identity of each cattle tank holding specific mesocosms
Temperature: Water temperature (°C)
Diff_temp: Difference between temperature in the morning versus in the afternoon (Temperature morning - Temperature afternoon) (°C)
Diff_temp_rel: Difference between temperature in the morning versus in the afternoon relative to the morning temperature ((Temperature morning - Temperature afternoon)/Temperature morning)
Oxygen_mgl: Disolved oxygen in the water (mg/L)
pH: pH of the water
Meto_level: Initial concentration of metolachlor (High = 80 µg/L, medium = 8 µg/L, low = 0.8 µg/L, nul = 0 µg/L) 
Drying_level: Drying rate (Rapid, medium, no-drying)

Metolachlor.csv

Date: Date at which a specific data was collected
JJ: Day at which data was collected
Bin_id: Identity of each mesocosm 
Conc_tot: Concentration of metolachlor measured in water samples
constante_k: k value to determine 1/2 life of metolachlor (Base on initial concention (t0) and concentration at time(t)
temps_vie: Half-life of metolachlor base on concentrations found in water samples
Drying_level: Drying rate (Rapid, medium, no-drying)
Meto_level: Initial concentration of metolachlor (High = 80 µg/L, medium = 8 µg/L, low = 0.8 µg/L, nul = 0 µg/L) 

In all data sheets, n/a, na, and NA indicate missing values or values that were not applicaple.
All statistical analyses were conducted in R version 4.1.2 (2021-11-01).
Platform: x86_64-w64-mingw32
Running under: Microsoft Windows 11 Home, Build 22621












